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Effect of Paclobutrazol on Water Stress-Induced Abscisic Acid in Apple Seedling Leaves

机译:多效唑对水分胁迫下苹果幼苗叶片脱落酸的影响

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摘要

Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) in water-stressed leaves from control apple seedlings, and also from apple seedlings treated for 28 days with paclobutrazol ([2RS, 3RS]-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazol-1-yl] pentan-3-ol). The ELISA quantitative estimates were also validated by gas chromatography-electron capture detector and lettuce seed germination inhibition bioassay. Paclobutrazol treatment reduced endogenous ABA levels by about one-third, and prevented the marked accumulation of water-stress-induced ABA that occurred in untreated seedlings. The presence of ABA in the apple leaf extracts was confirmed by gas chromatography-mass spectrometry.
机译:通过酶联免疫吸附测定(ELISA)对对照苹果幼苗以及用多效唑([2RS,3RS] -1- [4-氯苯基]处理28天的苹果幼苗的水分胁迫叶片中的脱落酸(ABA)进行定量] -4,4-二甲基-2- [1,2,4-三唑-1-基]戊烷-3-醇)。 ELISA定量估计值也通过气相色谱-电子捕获检测器和生菜种子发芽抑制生物测定法进行了验证。多效唑处理使内源性ABA水平降低了约三分之一,并防止了水分胁迫诱导的ABA在未经处理的幼苗中的显着积累。通过气相色谱-质谱法确认了苹果叶提取物中ABA的存在。

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